Background:: Serious infections by Pseudomonas aeruginosa are commonly treated with the combination of a beta-lactam antibacterial and an aminoglycoside. Therefore, production of a 16S rRNA methylase may result in an extremely important antibacterial resistance profile. Objectives:: The present study was conducted to determine the prevalence of Cephamycinase (CMY), aminoglycoside 6'-N-acetyltransferase (aac(6′)-Ib) and 16S rRNA methylase genes among Pseudomonas aeruginosa isolates from Iran. Patients and Materials:: This descriptive study was performed on hospitalized burnt patients during 2011and 2012. Antibiotics susceptibility tests were performed by disc diffusion and broth microdilution methods. CMY, aac(6′)-Ib, 16S rRNA methylase genes were detected by PCR method. Results:: Seventy-seven (77%) of 100 isolates were resistant to Imipenem and Ceftazidime. aac(6)-Ib, Cephamycinase (CMY), and rRNA methyltransferase (rmtB and rmtD) were detected in 57 (74.02%), 7 (9.09%), 11 (14.28%), and 9 (11.68%) isolates, respectively. PCR results for aminoglycoside resistance methyltransferase (armA) and rRNA methyltransferase (rmtC) were all negative. Aminoglycoside resistance methyltransferase (armA), and rRNA methyltransferase (rmtC) were not detected. Conclusions:: This study detected multiple drug resistance in Pseudomonas aeruginosa including resistance to β-lactams, Aminoglycosides, and Fluoroquinolones. Therefore, identification of drug resistance patterns in P. aeruginosa and detection of pan-resistant producing isolates are of great importance in prevention and control of infections in burn center ward.
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Jafari et al. (2013) studied this question.
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