Key result
Muscle excitability testing parameters showed no differences by sex, with small age-related differences appearing only in individuals older than 60 years.
Observational (n=197)
Muscle excitability testing parameters are not influenced by sex, and age only affects parameters reflecting muscle membrane potential from age 60 onwards, suggesting future studies do not need to control for sex in healthy control groups.
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Supports sex-pooled norms for muscle excitability testing; leaves open need for age-specific references beyond 60 years in prospective cohorts.
Objective: To establish normative data for muscle excitability testing in the tibialis anterior muscle of a healthy population, and to determine their dependence on age and sex. Methods: Parameters of muscle velocity recovery cycle recordings with 1, 2 and 5 conditioning stimuli of 197 healthy subjects and frequency ramp recordings of 151 healthy subjects were retrospectively analysed for age and sex differences. Results: There were no differences by sex and only small age differences were found in healthy subjects older than 60 years for the muscle excitability parameters muscle relative refractory period, early supernormality and latency to the first response in a train at 15 Hz and 30 Hz. Conclusions: In this study, based on a large sample of muscle velocity recovery cycle and frequency ramp recordings, we have provided normative data and shown that muscle excitability testing is not influenced by sex, and that age only has an influence from the age of 60 years onwards on parameters reflecting muscle membrane potential. Significance: Our results suggest that future studies no longer need to control for sex when using a healthy control group.
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Exl et al. (2025) conducted an observational in Healthy (n=197). Age and sex was evaluated on Muscle excitability parameters (muscle relative refractory period, early supernormality, and latency to the first response). Muscle excitability testing parameters showed no differences by sex, with small age-related differences appearing only in individuals older than 60 years.
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