S-adenosyl-L-methionine production by Saccharomyces sake. Optimization of the culture conditions for the production of cells with a high S-adenosyl-L-methionine content.

In studies on the production of S-adenosyl-L-methionine (AdoMet) by Saccharomyces sake Kyokai No. 6 in a bench-scale fermentor, the rate of growth of the organism in a mediumcontaining sucrose and urea as carbon and nitrogen sources, respectively, increased with a higher agitation speed (500rpm) or sucrose feeding, but the cellular content of AdoMetwas lower than that with a lower agitation speed (300rpm) or ethanol feeding.The additin of i -methionine was necessary for