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The NMR spectroscopy was used to determine a structure and physicochemical properties of liposomes prepared from L‐α‐phosphatidylcholine dipalmitoyl (DPPC) by the modified reverse‐phase evaporation method (mREV). 31P‐NMR study of the liposome in the temperature range from 340K to 350K allowed us to suggest that the single‐bilayered liposome has been obtained. Transmission electron micrographs (TEM) showed that the liposome vesicles, obtained with the use of the modified (mREV) method, were spherical in shape and in majority they were less than 100 nm in diameter.
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Pentak et al. (2006) studied this question.
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