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The methylation of newly synthesized ribosomal protein L11 has been obtained in an in vitro system using lambda rifd 18 DNA as template and S-adenosyl[3H]methionine as methyl donor. About four methyl groups are incorporated per mol of L11 synthesized and the bulk of the methyl groups are present in the protein as trimethyl-lysine. lambda rifd 18 DNA also contains the genes for rRNA and newly synthesized 16 S and 23 S RNA are also methylated in this in vitro system.
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Jerez et al. (1980) studied this question.
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