Anomeric form of the products from chitinase-catalyzed hydrolysis was analyzed by 1H-NMR spectroscopy. When the chitinase from Streptomyces griseus was added to the solution of substrate, N, N, N, N, N-pentaacetylchitopentaitol, β-anomer was first produced by the enzymatic hydrolysis, and then transformed to α-anomer by mutarotation. In contrast, the chitinase from Dioscorea opposita (yam) produced only α-anomer, which was transformed to β-anomer by mutarotation.
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Fukamizo et al. (1995) studied this question.