The reverse transcription polymerase chain reaction (RT-PCR) was successfully used to determine the occurrence of Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in field-grown apple cultivars. Both viruses were detected frequently in all 16 tested apple cultivars. As many as 27.86% ASPV-infected and 44% ASGV-infected trees were recorded among a total of 420 tested trees from 15 different orchards. Mixed infection with ASGV and ASPV was recorded in 16.7% of the trees.
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Jiban Kumar Kundu (2003) studied this question.
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