Codon Usage of Hepatitis E Viruses: A Comprehensive Analysis

Figures

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  Figure 1. Principal component analysis (PCA) based on the hepatitis E virus (HEV) complete coding sequences. The first dimension was plotted against the second dimension. PCA plot showed the deviations and similarity among the 59 synonymous codons of 98 HEV sequences included in the study. Seven HEV genotypes were presented by colors. The ellipses in the figure predicted new observations with a probability of 0.95. New observations from the same group were expected to fall inside the ellipses.

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  Figure 2. Dinucleotide abundance frequency based on the HEV complete coding sequences. The dashed lines showed overrepresented and underrepresented values. Seven HEV genotypes were presented by colors.

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  Figure 3. Parity Rule 2 (PR2) plot based on the HEV complete coding sequences. The center of the plot, where the value of both coordinates was 0.5, indicated no bias in mutation or selection rates. Seven HEV genotypes were presented by colors.

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  Figure 4. Effective number of codons (ENCs)-plot analysis based on the HEV complete coding sequences. ENC values were plotted against GC3s of the genotypes. The black line represented the standard curve when the codon usage bias was determined by only the GC3s composition. Seven HEV genotypes were presented by colors.

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  Figure 5. Neutrality analysis based on the HEV complete coding sequences. The correlation between GC content at first and second positions of codon (GC12s) and at third position of codon (GC3s) was calculated. The solid lines by colors represented the linear regression of GC12 against GC3s for the seven HEV genotypes. * Represented correlation significant at p < 0.05.