Gonadotrophin (GTH) was purified from the dogfish (Scyliorhinus canicula) by first preparing a glycoprotein fraction followed by chromatography on Con A-Sepharose and CM-cellulose.GTH and TSH activities were assayed using chicks and quail.Although CM-cellulose produced two peaks of GTH activity, their biological activities were similar, suggesting that the dogfish produces one GTH which is particularly LH-like.Introduction.While it is now widely accepted that two gonadotrophins, resembling mammalian LH and FSH, are produced by the pituitaries of birds, reptiles and amphibians, most work with bony fishes suggests the presence of only one gonadotrophin in this class of vertebrates (e. g.Burzawa-G6rQrd, 1971 ; Donaldson et al., 1972 ; Burzawa- G6rard et al.,1975 ; Breton et al., 1976).This conclusion is, however, being questioned in recent publications (e. g.Idler et a/., 1975 ; Pierce et ai., 1976 ; Farmer and Papkoff, 1977).No information exists on the gonadotrophin(s) of the cartilaginous Elasmo- branch fishes and the present study presents some results on the purification of this hormone from the lesser spotted dogfish (Scyliorhinus canicula).
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Sumpter et al. (1978) studied this question.