A method of purifying trout gonadotropic hormone is discussed.The technique consists of the following stages : alcoholic extraction, two successive gel filtrations on Sephadex G-5 o and G-ioo, chromatography on DEAE-cellulose and ultrogel ACA S ¢.The biological activity of fractions obtained is measured using as assay the maturation in vitro of trout oocytes.The preparation obtained has a molecular weight of about 27500 , determined by behavior on Sephadex-G 100 and analytical ultracentrifugation for a KS $ow of z.¢ 5 .Polyacrylamide gel electro- phoresis shows two strips of electrophoretic mobility, 0 .50 and 0 .5 8. Analysis of the amino acid composition of the preparation indicates a number of 1 6 0 AA residues per mole.These different values are compared to the corresponding ones for c-GtH, s-GtH and mammalian gonadotropins.2 .-Gel filtration on Sephadex EPH is fractionated by Sephadex G-50 gel filtration on a 6 0 X 2 .5 cm column with Tris-HCI 0 .05 M buffer, pH 7 .8for the first preparation; the same buffer of o.oim molarity at pH 8. 0 , containing 0 .5 mM dithiothreitol, is used for the second preparation.
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Breton et al. (1976) studied this question.