Virus Genome Sequences in the Blood of Myalgic Encephalomyelitis/Chronic Fatigue Syndrome Patients

Figures

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  Figure 1.. Molecular probe technology. (A) Molecular probe design. The 60-base Homer is split into two end segments of 30 bases each. The central section of 36 bases is common to all probes and is complementary to the 20-mer forward and reverse primers that overlap by four bases at their respective 5’ ends. The green color on the left end represents a 5’ phosphate group. The red color on the right end represents a 3’ hydroxyl group. (B) Probe hybridized to its target. When a probe is hybridized to its target, the 5’-phosphate is adjacent to the 3’-hydroxyl group. No bases are missing. Therefore, the enzyme DNA ligase will synthesize a phosphodiester bond to create a circular, single-stranded DNA.

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  Figure 2.. Virus sequences detected in each clinical sample. This figure presents a heatmap of those molecular probes which are significantly enriched. The rows correspond to individual probes. The right labels present the virus name. The columns correspond to the individual clinical samples. The top color bar indicates the group (blue, negative controls [water reacted with the probe ensemble; no DNA present]; pink, ME/CFS patients; green, healthy controls.) The bottom labels give the identification of each clinical sample. Cell colors indicate the presence or absence of the molecular probe in the sample (red, presence; white, absence).