Addition of formaldehyde to ammonium sulfate extracts of myoglobin from human muscle increased the stability and the antigenicity of the myoglobin. Antiserum to this preserved extract does not react with hemoglobin. Immunologic tests of formaldehyde-preserved urine indicated that myoglobin or a myoglobin-like substance was present in all urines tested. Urines containing bile and urines from patients with recent myocardial infarctions contained larger amounts of the immunologically reactive substance.
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Strausser et al. (1966) studied this question.