Key result
Curcumin partially attenuates doxorubicin-induced oxidative stress and myocyte apoptosis, peaking at 12 mg/L.
Why the study?
The effects of curcumin on phosphate carrier expression and its protective role against doxorubicin-induced cardiomyocyte toxicity were not well understood.
Does curcumin prevent doxorubicin-induced cardiomyocyte apoptosis and oxidative stress in H9c2 cells?
Does curcumin prevent doxorubicin-induced cardiomyocyte apoptosis and oxidative stress in H9c2 cells?
Curcumin demonstrates protective effects against doxorubicin-induced cardiotoxicity in vitro by downregulating phosphate carrier expression and reducing oxidative stress and apoptosis.
May support curcumin investigation in doxorubicin cardiotoxicity; hypothesis-generating from cellular data.
Aim. To explore the effects of curcumin on phosphate carrier (PiC) and its role in protection against doxorubicin induced myocyte toxicity. Methods. Using H9c2 cell line, the cardiotoxic effect of doxorubicin and its mitigation by curcumin were studied. H9c2 cells were cultured with doxorubicin and/or curcumin at various concentrations. Analysis for apoptosis of H9c2 was done using flow cytometry. Confocal laser scanning microscopy was used to record the fluorescence intensity ratios and to determine the mitochondrial permeability transition pore (MPTP) opening state. Oxidative stress was measured using glutathione level, superoxide dismutase activities, and malondialdehyde content. The effect of doxorubicin and curcumin on PiC gene expression was measured by real-time PCR. Results. Curcumin decreased mRNA of PiC and was partly protective against oxidative stress, loss of mitochondrial transmembrane potential, and apoptosis induced by doxorubicin. Interestingly, the effect was not clearly dose dependent and the concentration of 12 mg/L was more efficient than 15 and 10 mg/L. Conclusion. Curcumin downregulates PiC and partly protects against doxorubicin induced oxidative stress and myocyte apoptosis.
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Junkun et al. (2016) studied Doxorubicin-induced cardiomyocyte apoptosis. Curcumin vs. Doxorubicin alone was evaluated on Phosphate carrier (PiC) gene expression, oxidative stress, and apoptosis. Curcumin downregulates phosphate carrier and partly protects against doxorubicin-induced oxidative stress and myocyte apoptosis, with 12 mg/L being more efficient than 10 or 15 mg/L.
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