Amino Acid Phosphoramidate Nucleotides as Alternative Substrates for HIV‐1 Reverse Transcriptase

Pick and choose: Amino acid phosphoramidites, in particular Asp-dAMP (dAMP=2′-deoxyadenosine-5′-monophosphate) and His-dAMP, act in a template-dependent DNA synthesis assay as alternative substrates for reverse transcriptase. L-Aspartic acid can function as a leaving group during DNA synthesis and can be considered as a pyrophosphate mimetic in this reaction.