Key result
Trichosporon asahii infection of THP-1 cells altered the expression of 46 circRNAs, 412 mRNAs, and 47 miRNAs at 12 hours, with hsa_circ_0065336 regulating PTPN11 via miR-505-3p sponging.
This study identifies a circRNA-associated ceRNA network involved in T. asahii infection, offering new insights into host-pathogen interactions.
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Should not alter T. asahii management; leaves open ceRNA network as hypothesis-generating target in host-pathogen interactions.
BACKGROUND: Invasive Trichosporon asahii (T. asahii) infection frequently occurs with a high mortality in immunodeficient hosts, but the pathogenesis of T. asahii infection remains elusive. Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that participate in various disease processes. However, the mechanism of circRNAs in T. asahii infection remains completely unknown. METHODS: RNA sequencing (RNA-seq) was performed to analyze the expression profiles of circRNAs, microRNAs (miRNAs), and mRNAs in THP-1 cells infected with T. asahii or uninfected samples. Some of the RNA-seq results were verified by RT-qPCR. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were used to analyze the differentially expressed mRNAs. A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments. RESULTS: A total of 46 circRNAs, 412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T. asahii infection. GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response, the Toll-like receptor signaling pathway, and the TNF signaling pathway. A competing endogenous RNA (ceRNA) network was constructed with 5 differentially expressed circRNAs, 5 differentially expressed miRNAs and 42 differentially expressed mRNAs. Among them, hsa_circ_0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p. CONCLUSIONS: These data revealed a comprehensive circRNA-associated ceRNA network during T. asahii infection, thus providing new insights into the pathogenesis of the T. asahii-host interactions.
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Zhang et al. (2021) studied Trichosporon asahii infection. Trichosporon asahii infection vs. Uninfected samples was evaluated on Differential expression of circRNAs, miRNAs, and mRNAs. Trichosporon asahii infection of THP-1 cells altered the expression of 46 circRNAs, 412 mRNAs, and 47 miRNAs at 12 hours, with hsa_circ_0065336 regulating PTPN11 via miR-505-3p sponging.
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