Pd22-03 Collagenase Clostridium Histolyticum Degrades Type I and Iii Collagen While Sparing Type Iv Collagen in Vitro in Peyronie’s Plaque Explants

You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Peyronie's Disease1 Apr 2014PD22-03 COLLAGENASE CLOSTRIDIUM HISTOLYTICUM DEGRADES TYPE I AND III COLLAGEN WHILE SPARING TYPE IV COLLAGEN IN VITRO IN PEYRONIE’S PLAQUE EXPLANTS Laurence A. Levine, Tom M. Schmid, Susan G. Emeigh Hart, Thomas Tittelbach, Michael P. McLane, and James P. Tursi Laurence A. LevineLaurence A. Levine More articles by this author , Tom M. SchmidTom M. Schmid More articles by this author , Susan G. Emeigh HartSusan G. Emeigh Hart More articles by this author , Thomas TittelbachThomas Tittelbach More articles by this author , Michael P. McLaneMichael P. McLane More articles by this author , and James P. TursiJames P. Tursi More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.1854AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Collagen types I and III are the predominant collagen types in Peyronie’s disease (PD) plaques. Collagenase clostridium histolyticum (CCH) has been studied in clinical trials for treatment of PD in adults. Our objective was to examine the effect of CCH on PD plaque tissue regarding temporal pattern, dose dependency, and subtype selectivity of collagen lysis, and histomorphological evaluation of collagen lysis and surrounding tissue. METHODS PD plaques from men (n=4) undergoing corrective surgery were obtained ≤1 hour after excision. Plaque tissue explants were injected with CCH (0-3000 U; equivalent to 0-0.174 mg) and incubated for 2, 4, or 12 hours; collagenolytic activity of types I, III, and IV were evaluated using immunohistochemistry at 12 hours post-injection. RESULTS Collagen lysis by CCH was selective for Types I and III collagen, as evidenced by focal to diffuse loss of immunostaining for one or both components in injected tissues (Figure). Type IV collagen associated with blood vessel/capillary basement membranes, smooth muscle, and the perineurium/epineurium of nerves was unaffected, except at the highest dose of CCH (3000 U) and longer incubation times. There was no detectable effect of CCH on the histomorphology of blood vessels, nerves, and fibroblasts at any dose or incubation timepoint evaluated. CONCLUSIONS Consistent with previous reports, these findings indicate direct collagenolytic activity of CCH on PD plaque tissue explants in vitro. These data suggest selectivity of CCH for types I and III collagen, the predominant collagen types in PD plaques, and sparing of type IV collagen, which is present within connective tissues that surround arteries, large veins, and nerves. Immunohistochemistry results support previous reports of preserved functional integrity of arteries and nerves. These findings support a mechanistic basis for the collagenolytic activity of CCH in PD plaque explants without impairment of nerves, blood vessels, or large veins. © 2014FiguresReferencesRelatedDetails Volume 191Issue 4SApril 2014Page: e672-e673 Advertisement Copyright & Permissions© 2014MetricsAuthor Information Laurence A. Levine More articles by this author Tom M. Schmid More articles by this author Susan G. Emeigh Hart More articles by this author Thomas Tittelbach More articles by this author Michael P. McLane More articles by this author James P. Tursi More articles by this author Expand All Advertisement Advertisement PDF DownloadLoading ...