Affinity chromatography-purifled elderberry (Sambucus nigra L.) seed proteins strongly inhibited protein synthesis and displayed the 28S rRNA N-glycosidase activity characteristic of all types of ribosome-inactivating proteins (RIPs). Western blot analysis revealed several proteins that reacted with antibodies raised against the novel non-toxic type 2 ribosome-inactivating protein nigrin b isolated from elder bark, thus indicating the presence of a new type 2 RIP.
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Citores et al. (1994) studied this question.
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