Changes in Bovine Milk Secretion Following Intramammary Infusions of Concanavalin A, Oyster Glycogen, or Water

Effects of sterile intramammary infusion of Concanavalin A on milk secretion were contrasted with infusion of oyster glycogen or water. Twenty-four cows were infused intramammary with 100 mg Concanavalin A, oyster glycogen in 20 ml water, or with 20 ml water alone. Concentrations of lactose, somatic cells, immunoglobulins G and A, serum albumin, and activity of N-acetyl-B-D-glucosaminidase were determined in milk. Blood N-acetyl-B-D-glucosaminidase activity and concentrations of blood immunoglobulins G and A and serum albumin were determined. Oyster glycogen and concanavalin A caused inflammation in treated quarters; peak elevations of milk somatic cell counts, serum albumin, immunoglobulin G concentrations, and N-acetyl-B-D-glucosaminidase activity were at 12 to 36 h following treatment. Milk production and lactose concentration were reduced by oyster glycogen and Concanavalin A. Selective indices of relative accumulation of milk immunoglobulins decreased following Concanavalin A and oyster glycogen, whereas the N-acetyl-B-D-glucosaminidase activity selective index generally remained unchanged. Inflammation reduced the selective accumulation of immunoglobulins, and absence of change in the N-acetyl-B-D-glucosaminidase selective index indicated that blood is not a major source of milk N-acetyl-B-D-glucosaminidase.