Glucose Oxidation by Modified Mould Mycelium

A new strain of Aspergillus niger containing a high intracellular glucose oxidase and catalase activity could be isolated. The glucose oxidase of this strain showed some favourable properties with regard to enzymatic glucose oxidation in industrial scale. The mycelium was permeabilized by treatment with iso‐propanol. Then, additional catalase was bound to the mycelium so that a coimmobilizate, resulted. The enzymatic characteristics of this coimmobilizate, as compared with former immobilized glucose oxidase/catalase preparations, showed higher temperature stability and lower Km‐value for glucose. Some basic trials were done for glucose oxidation in a stirred tank reactor where oxygen supply was carried out by controlled addition of hydrogen peroxide instead of conventional aeration. The influence of temperature on the stability of both, glucose oxidase and catalase, was investigated under these application conditions.