Key result
Novel trout myogenin homolog expresses during developing myotomal musculature and in vitro myosatellite cell fusion.
Why the study?
A gene with homology to myogenin had not been identified or characterized in teleost fish such as rainbow trout, and its role in muscle differentiation was unclear.
Key points are not available for this paper at this time.
Design
Molecular cloning and in vitro myogenesis study
Answers are grounded in this paper and cite their sources.
Trout myogenin homolog expressed during myogenesis; extends teleost models but leaves open mammalian translation.
We report the cloning of a new trout myogenic cDNA which encodes helix-loop-helix protein homologous to the myogenic factor myogenin. Northern analyses indicate that trout myogenin (Tmyogenin) transcripts accumulate in large amounts in the myotomal musculature of embryos and frys. In adults, transcripts concentrate within the thin lateral layer of red (slow oxydative) muscle fibres. They are present only in low amounts in white (fast glycolytic) muscle fibres which constitute the major part of the trunk musculature. Using an in vitro myogenesis system, we observed that the trout myogenin encoding gene is not activated until myosatellite cells fuse to generate multinucleated myotubes, indicating that Tmyogenin lies downstream of muscle determination factors. All these observations show that in a major taxinomic group like teleosts, a gene with homology to myogenin exists. Its activation during myogenesis suggests that it acts as a major developmental regulator of muscle differentiation.
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Rescan et al. (1995) studied this question. A new trout myogenic cDNA homologous to myogenin was cloned and found to be expressed in developing myotomal musculature and during in vitro myosatellite cell fusion.
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