Identification of key pathways and genes in miR-30e regulating osteogenesis in aortic smooth muscle cells using bioinformatics analysis

Background: Cardiovascular disease (CVD) is an important problem that threatens the health of all mankind and remains the world's first cause of morbidity and mortality.In this study, we identified the gene characteristic during vascular calcification and explored their potential mechanisms.Results: Gene expression profiles of GSE65435 were downloaded from GEO database.The GSE65435 dataset contained 18 samples, the 3 SMCs + miR-30e samples and 3 SMCs + ct-miR samples were selected.The gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) enrichment analyses were implemented, and gene signal network and pathway relation network of the differentially expressed genes (DEGs) were analyzed by GCBI.In total, 620 differentially expressed genes were identified in SMCs + miR-30e samples, including 249 up-regulated genes and 371 down-regulated genes.GO analysis summed up that DEGs were significantly concentrated in negative regulation of apoptotic process.KEGG pathway analysis showed that DEGs were enriched in PI3K-Akt signaling pathway.Conclusions: This study showed that the identified DEGs increased the molecular mechanisms underlying the development of vascular calcification and might be used as new diagnostic and therapeutic strategies for the treatment of vascular calcification.