Key result
Novel VP1-targeted RT-PCR assay enables broad-range molecular serotyping of porcine teschoviruses and enteroviruses.
Why the study?
Difficulties in serological detection of porcine enteroviruses and teschoviruses may lead to underestimation of infections with clinical symptoms, necessitating molecular diagnostic approaches.
A new RT-PCR assay targeting the VP1 region provides a useful diagnostic tool for molecular serotyping of porcine teschoviruses and enteroviruses.
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May improve PTV/PEV detection in swine; leaves open clinical validation of molecular serotyping.
Rosa et al. (2006) studied Porcine enteroviruses and teschoviruses (n=43). RT-PCR assays targeting the VP1 coding region was evaluated on Serotype characterization and molecular diagnosis. A newly designed RT-PCR assay targeting the VP1 region proved suitable for broad-range serotype assessment and molecular diagnosis of porcine teschovirus and enterovirus strains.
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