Key result
Promoter prediction and transcriptome profiling successfully identified the transcription start sites and active regulatory elements for the 90-kDa, 140-kDa, and 200-kDa palladin isoforms in mouse myogenic cells.
This study identifies the transcription start sites and regulatory elements of the palladin gene promoter, contributing to the understanding of its role in myogenesis.
Preclinical palladin promoter mapping in mice warrants no clinical change; leaves open isoform roles in human myogenesis or cardiomyopathy.
OBJECTIVE: Palladin is a ubiquitous phosphoprotein expressed in vertebrate cells that works as a scaffolding protein. Several isoforms deriving from alternative splicing are originated from the palladin gene and involved in mesenchymal and muscle cells formation, maturation, migration, and contraction. Recent studies have linked palladin to the invasive spread of cancer and myogenesis. However, since its discovery, the promoter region of the palladin gene has never been studied. The objective of this study was to predict, identify, and measure the activity of the promoter regions of palladin gene. RESULTS: By using promoter prediction programs, we successfully identified the transcription start sites for the Palld isoforms and revealed the presence of a variety of transcriptional regulatory elements including TATA box, GATA, MyoD, myogenin, MEF, Nkx2-5, and Tcf3 upstream promoter regions. The transcriptome profiling approach confirmed the active role of predicted transcription factors in the mouse genome. This study complements the missing piece in the characterization of palladin gene and certainly contributes to understanding the complexity and enrollment of palladin regulatory factors in gene transcription.
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Ouali et al. (2020) studied Skeletal muscle cell maturation. Palladin promoter constructs vs. Empty vector control was evaluated on Luciferase reporter activity and mRNA expression. Promoter prediction and transcriptome profiling successfully identified the transcription start sites and active regulatory elements for the 90-kDa, 140-kDa, and 200-kDa palladin isoforms in mouse myogenic cells.
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