Key result
Actinomycin D reduced the synthesis of echovirus 12, its RNA, and polypeptides, and decreased the proportion of virus particles to empty capsids.
Actinomycin D inhibits the synthesis of echovirus 12 and alters the ratio of complete virus particles to empty capsids.
Echovirus polypeptide profiles extend picornavirus assembly models; leaves open any clinical diagnostic or therapeutic relevance.
Electrophoretic analysis of purified echovirus virus particles yielded four polypeptides of mol. wt. 37000, 30000, 25000 and 7600. The 75S empty capsids of echovirus 12 lack the 25000 and 7600 mol. wt. polypeptides, and possess polypeptides of 41000 mol. wt. A total of 14 virus-induced polypeptide species was found in the cytoplasm of infected cells. Actinomycin D reduced the synthesis of virus, virus RNA, and virus polypeptides and also reduced the proportion of virus particles to empty capsids in the virus yields.
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Korant et al. (1975) studied Echovirus 12 infection (in vitro). Actinomycin D was evaluated on Synthesis of virus, virus RNA, and virus polypeptides. Actinomycin D reduced the synthesis of echovirus 12, its RNA, and polypeptides, and decreased the proportion of virus particles to empty capsids.
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