Primers complementary to conserved sequences of cucumber mosaic virus (CMV) RNA 3 were designed and reverse transcription‐polymerase chain reaction was directly performed in crude sap extracts of CMV‐infected plants. All isolates assayed belonging to subgroups I or II from different Spanish geographical locations and infecting tobacco, tomato and cucumber plants were detected. No amplification was observed in healthy or tomato aspermy virus (TAV)‐infected plants, nor in CMV‐infected pepper plants.A, dilution as low as 10‐7 in tobacco crude sap extracts or 100 fg of purified virus (c. 50 000 virus particles) could be detected, results showing that this procedure is 102 times more sensitive than enzyme‐linked immunosorbent assay (ELISA).
No takes yet. Share an insight, caveat, or question.
Blas et al. (1994) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: