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January 1, 2023Biotechnology Research and InnovationOpen Access

Real-Time PCR Assay for detection and quantification of Leishmania: standardization, positive control, validation, and intra-laboratory assay

Manuel Hospinal-Santiani Carlos Ricardo Soccol Susan Grace Karp Eduardo Scopel Ferreira da Costa Luiz Alberto Júnior Letti Germana Dávila dos Santos Vanete Thomaz‐Soccol

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Abstract

Highlights• In this work is presented product and process for the diagnosis and quantification of Leishmania parasite load by PCR and qPCR • The method sensitivity was determined as 0.01 parasites/μL • The method has 100% concordance among human participants in the intra-laboratory validation study

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Cite This Study

Hospinal-Santiani et al. (2023) studied this question.

synapsesocial.com/papers/6ab9b5651a8f5908747bc91e https://doi.org/10.4322/biori.00062022

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