Dataset and analysis scripts for: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM Study

This repository contains the raw data and analysis scripts supporting the manuscript below. Manuscript: Fluorescence-based Size Determination Improves Reproducibility over Light Scattering in Nanoparticle Tracking Analysis of Extracellular Vesicles: A Correlative AFM/SMFM StudyTarget journal: Nanoscale (RSC)Corresponding author / data contact: jaroslaw.jacak@fh-linz.at It includes raw AFM-QI data (.gwy), correlative fluorescence microscopy images, ROI sets used for spot localization, NTA export files, and the Python scripts used for data processing, colocalization analysis, and figure generation. Folder structure and file naming are described in the accompanying README. Raw data files are licensed under CC-BY 4.0; analysis scripts (.py) are licensed under the MIT License.