Immunological analysis of incompatibility (S) proteins and of cross-reacting material in a self-compatible mutant ofOenothera organensis

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Proteins of pollen ofOenothera organensisfrom heterozygousS2S3,S2S6,S3S6andS4S6and homozygousS6S6genotypes produce characteristic precipitation lines in agar-gel plates against antisera prepared from the pollen. The maximum number of precipitation lines was three, and with some genotypes only one line was formed. The lines were characteristic of the differentSalleles, but there was a strong cross-reaction betweenS4pollen andS6antiserum. TheSprotein diffused out from intact pollen grains as readily as from macerated pollen indicating that the normal site of action of theSprotein, when the pollen tubes are growing down the style, is near the surface of the pollen tube. The self-compatible mutantS6′S6in which theS6′allele has lost the ability to produce an active pollenSprotein, has a protein which cross-reacts strongly withS6antiserum.