A stomatal inoculation apparatus (SIA) was developed to produce water congestion of leaf tissues and provide a reproducible noninjurious means of introducing two Xanthomonas campestris pathovars of citrus into leaf tissues without wounding. The SIA consisted of a small inoculation chamber attached to an intact leaf. Water and inoculum were metered into an airstream and focused to impact on a 1-mm-diameter area of the leaf surface. Leaf tissues on the abaxial surface of Duncan grapefruit leaves expanded 50-75% were more susceptible to infection than were other growth stages (...)
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T. R. Gottwald (1992) studied this question.