Virucidal Activities of Common Disinfectants Against the Japanese Eel Endothelial Cells-Infecting Virus (JEECV)

In this study, we investigated the virucidal activities of common disinfectants against the Japanese eel endothelial cells-infecting virus (JEECV) in vitro using JEE-2, a Japanese eel vascular endothelial cell line. After treatment with different concentrations of each disinfectant, virus suspension was added to the cells in 96-well plates and incubated for 10 days at 25°C. No cytopathic effects were observed after 1-min treatment with ≥50% ethanol, ≥20 ppm sodium hypochlorite, ≥50 ppm povidone-iodine, ≥500 ppm cresol, and ≥1,000 ppm benzalkonium chloride. These results suggest that appropriate disinfection controls the spread of JEECV infection on eel farms.