Key result
Aortic stenosis linked to ~74% higher MMP9 mRNA levels compared with aortic regurgitation.
Why the study?
Differential diagnostics of aortic valve stenosis and aortic regurgitation using molecular techniques were needed to improve diagnostic effectiveness.
Case-Control (n=93)
Effect estimate: 74% and 87% higher respectively
p-value: p=<0.01 and <0.001
May support molecular differentiation of AS from AR; leaves open prospective validation before clinical adoption.
OBJECTIVE: Aortic valve stenosis (AS) presents a disease during which there are changes of the aortic valve structure that modify the blood structure of patients. The aim of this study was to improve the effectiveness of differential diagnostics of aortic stenosis and aortic regurgitation using molecular techniques on both mRNA (RT-PCR) and protein (biochip protein). PATIENTS AND METHODS: An experimental group (n = 58) consisting of patients with aortic valve stenosis (n = 26) and aortic regurgitation (AR, n = 32) was compared with a control group (n = 35). Both blood serum and valve tissue samples were used for the determination of gene expression specific genes related to inflammatory response (CRP, IL6, IL2R, IL6R, TNFR1, and 2) as well as genes and proteins involved in remodeling of the extracellular matrix (MMP9, TIMP, Emilin-1). RESULTS: We found that hsCRP and IL6 plasma levels of patients with AS were higher than both controls and patients with AR (mean 5.6 ng/ml). The differences between AS and AR were detected only in mRNA levels of MMP9 and TIMP where increased levels characteristic for AS were found (about 74%, p < 0.01 and 87%, p < 0.001 higher than AR). CONCLUSIONS: The achieved results could contribute to the improvement of early diagnosis of selected cardiovascular disease in the future and improve the quality of patient's life.
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Urban et al. (2019) conducted a case-control in Aortic valve stenosis and aortic regurgitation (n=93). Aortic valve stenosis vs. Aortic regurgitation and healthy controls was evaluated on Differences in mRNA levels of MMP9 and TIMP (74% and 87% higher respectively, p=<0.01 and <0.001). Patients with aortic valve stenosis had significantly higher mRNA levels of MMP9 (74% higher, p<0.01) and TIMP (87% higher, p<0.001) compared to patients with aortic regurgitation.
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