IMO, a commercially available mixture of isomaltooligosaccharides, was fractionated by preparative HPLC, and two fractions (IM02 and IM03) were obtained. IM02 contained mainly disaccharides (86.4%), which IM03 contained tri- and higher oligosaccharides (89.9%). The administration of IM02 or IM03, ranging in amount from 5 to 20g/day, increased human intestinal bifidobacteria in dose-dependent manner. An IM02 intake of 10 g/day and IM03 of 5 g/day each produced a significant increase of bifidobacterial number in feces and the ratio in fecal microflora within 12 days.
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Kaneko et al. (1994) studied this question.