LC-MS/MS Content Analysis of Ethanol Extracts from Some Naturally Occurring Plants and Their Biological Activity Against Certain Plant Pathogenic Microorganisms Under In Vitro Conditions

There is a significant need for environmentally friendly organic pesticides. This study was conducted to analyze the content of ethanol extracts from some naturally occurring plant parts in Elazığ, Türkiye, using LC-MS/MS and to determine their activity against certain plant pathogenic bacteria and microfungi. The study was carried out under in vitro conditions using a Randomized Complete Block Design with four replications. Antibacterial activity was assessed using the Agar Well Diffusion Method in Mueller-Hinton medium, while antifungal activity was determined using the Poisoned Food Method in Malt Extract Agar medium. The statistical analysis of the obtained data sets was performed using the Kruskal-Wallis H test. As a result, among the 33 standard compounds investigated in the extracts, none were found in Asphodelus aestivus L. and Melissa officinalis; three compounds vanillin, gentisic acid, chlorogenic acid) were identified in Tanacetum parthenium; and four compounds vanillin, trans-cinnamic acid, p-coumaric acid, hydroxybenzaldehyde) were identified in Rosa canina L. Rosa canina L. extract showed no activity against the pathogenic test microfungi, while the activity of Tanacetum parthenium extract increased with higher concentrations. It was observed that the extracts of Asphodelus aestivus L. and Melissa officinalis completely inhibited the growth of microfungi at concentrations of 15% and above(v/v).