Nemafric-BL phytonematicide induces egg hatch inhibition inMeloidogyne incognita

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Contribution of egg hatch inhibition in the overall reduction of Meloidogyne incognita numbers by Nemafric-BL phytonematicide was determined in vitro using a series of water-diluted phytonematicide solutions. Hatched juveniles were assessed at four different incubation periods. Thereafter, each phytonematicide solution was water-diluted 5 times and incubated for 5 days to evaluate the reversibility of egg hatch inhibition. At all exposure periods, except the shortest (24 h), concentration effects on egg hatch were highly significant with juvenile numbers versus increasing concentrations exhibiting quadratic relations. The models for the three consecutive exposure periods explain the quadratic relations by 95%, 94% and 98%, respectively. Results suggested that egg hatch inhibition was one of the mechanisms involved in reducing M. incognita population densities by Nemafric-BL phytonematicide.