sion (Contract AT(45-1)-343) and United States Public Health Service (Contract H-1179 (Cl).2 We are indebted to Smith, Kline, and French Labora- An hemolysate of human ACD blood was prepared by rapid freezing and thawing of the sample four times, and the stroma fraction was removed by the method of Pen- nell and Smith (2).When a bloodl sample containing hemolysate and stroma was centrifuged, preparatory to erythrocyte analysis, the stroma sedimented above the leucocyte layer and could be easily removed.The post-transfusion survival of human erythrocytes was determined by the method of Young, Platzer, and Rafferty (3) with slight modifications.Baseline inag- glutinable cell counts of 5,000 to 30,000 and of 4,000 to
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Gabrio et al. (1954) studied this question.
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