Key result
Taurocholate reduces VLDL triacylglycerol secretion by ~80% in isolated rat hepatocytes.
Why the study?
The effect of bile acids, specifically taurocholate, on VLDL triacylglycerol synthesis and secretion by hepatocytes was unclear.
Caution against clinical extrapolation; leaves open in vivo relevance of bile acid effects on VLDL secretion.
The influence of taurocholate on very low density lipoprotein (VLDL) triacylglycerol synthesis and secretion was studied by isolated rat liver-parenchymal cells. The incorporation of [3H]glycerol into cell-associated and VLDL triacylglycerols were measured after incubation in medium containing 0.75 mM oleate. Taurocholate caused a maked decrease in VLDL [3H]triacylglycerol secretion from the hepatocytes: 50-150 microM taurocholate inhibited secretion of VLDL [3H]triacylglycerols by 70-90%. Similar results were obtained when the mass of secreted VLDL triacylglycerols was measured. Taurocholate caused a decreased secretion of VLDL [3H]triacylglycerols after 15-30 min incubation. A higher amount of cellular triacylglycerols was found in taurocholate-supplemented cells. Furthermore taurocholate did not change the intracellular lipolysis of triacylglycerols. These results suggest that bile acids interfere more probably with the assembly and/or secretion of VLDL-particles and not with earlier stages of VLDL formation, e.g. triacylglycerol synthesis.
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Pozo et al. (1987) studied this question. Taurocholate (bile acids) was evaluated on Secretion of VLDL [3H]triacylglycerols (70-90% inhibition). Taurocholate (50-150 microM) inhibited the secretion of VLDL triacylglycerols by 70-90% in isolated rat hepatocytes, suggesting interference with VLDL assembly and/or secretion.
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