Identification and Expression Characteristic Analysis of CML Gene Family of Melon

Calmodulin-like ( CML ) is one of the important Ca 2+ sensors, which plays an important role in plant growth and development and stress response. In this study, melon ( Cucumismelo L.) genomic data was used to identify the melon CML protein family using bioinformatics methods, and its physical and chemical properties, location information, gene structure, phylogeny, and promoter were analyzed. The results showed that 60 CmCML protein genes were identified in the melon genomic data, containing 1~4 EF-hand domains, which were unevenly distributed on 12 chromosomes. By analyzing the evolutionary treewith Arabidopsis CMLs , CmCML could It is divided into 8 groups, and the number of various groups is different. It has certain similarities with other plant CML family genes in group classification. In the promoter analysis, abscisic acid, jasmonic acid, auxin, and gibberellin were identified. And drought, low temperature, mechanical damage and other signal response homeopathic elements. Real-time fluorescence quantitative results showed that gene expression of melon CML protein family was induced by the specialization of Fusarium oxysporum, these results predicted that the transcriptional regulation of melon CML protein family may participate in the resistance response of melon wilt, which is the gene family. The functional identification of each member laid a theoretical foundation.