The structure of the core region of the lipopolysaccharide from Klebsiella pneumoniae O3

The structure of lipid A–core region of the lipopolysaccharide (LPS) from Klebsiella pneumoniae serotype O3 was determined using NMR, MS and chemical analysis of the oligosaccharides, obtained by mild acid hydrolysis, alkaline deacylation, and deamination of the LPS:where P is H or α‐Hep; J is H or β‐GalA; R is H or P (in the deacylated oligosaccharides).Screening of the LPS from K. pneumoniae O1, O2, O4, O5, O8, and O12 using deamination showed that they also contain α‐Hep‐(1→4)‐α‐Kdo‐(2→6)‐GlcN and α‐Kdo‐(2→6)‐GlcN fragments.