Porcupine‐Quill‐Inspired Shape‐Morphing Biointerfaces Disassemble Biofilms

Mature biofilms are difficult to eradicate because the extracellular polymeric substance (EPS) matrix shields embedded bacteria from chemical and mechanical attack. Here, we report a porcupine‐spine‐inspired photo‐responsive dynamic offensive bio‐interface that dismantles mature biofilms through electrically actuated mechanical penetration and light‐driven oxidative stress. A high‐aspect‐ratio nano‐dagger array undergoes reversible nanoscale deformation (∼110 nm) under an alternating electric field, while a photo‐responsive heterostructure generates a surface photovoltage (∼364 mV) that enhances reactive oxygen species production. Mechanical penetration continuously disrupts the biofilm–surface interface, exposing bacteria within the EPS matrix to oxidative attack. This synergistic mechanism enables nearly complete biofilm eradication under complete immersion in bacterial suspensions of 10 9 CFU mL −1 , outperforming representative state‐of‐the‐art systems while maintaining stable antibiofilm activity for at least 10 days without chemical biocides. This work establishes a dynamic offensive strategy for durable self‐cleaning and antifouling interfaces.