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Six preparations of egg yolk media inoculated with Escherichia coli (Migula) were evaluated as an in vitro growth system for immature house flies, Musca domestica L. Larval development, based on pupation and eclosion success, was normal on all media supplemented with E. coli, but few larvae survived without bacteria (4%). Pupation (72%) and eclosion (63%) were significantly higher on egg yolk media without mannitol than on media with mannitol or on blood agar controls.
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Watson et al. (1993) studied this question.
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