A simple reproducible method for the ultramicro determination of total and direct serum bilirubin is presented. This method provides a greater sensitivity than that obtained with other colorimetric ultramicro procedures. Total bilirubin values agree with those obtained with the macro Malloy-Evelyn procedure, but direct-reading bilirubin values were significantly higher with the presented method.
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Rutkowski et al. (1966) studied this question.
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