Key result
Tissue Mapper software enables 3D anatomical reconstruction and single-cell mapping of intrinsic cardiac neurons.
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Enables detailed neuroanatomical mapping in rodent models; leaves open translation to human cardiac innervation studies.
This protocol describes the process of using the Tissue Mapper software to map single neurons of the intrinsic cardiac nervous system, annotate or "trace" key cardiac anatomy on select histological sections of an image volume, and to visualize the mapped neurons and traced anatomy in a 3D reconstructed heart.
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Leung et al. (2020) studied this question. Tissue Mapper software was evaluated. Tissue Mapper software enabled the single-cell mapping of intrinsic cardiac neurons and the 3D anatomical reconstruction of rat hearts from histological image stacks.
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