(−)- trans -Δ 9 -Tetrahydrocannabiphorol Content of Cannabis sativa Inflorescence from Various Chemotypes

The (−)- trans -Δ 9 -tetrahydrocannabiphorol (Δ 9 -THCP, 1 ) content of the inflorescence from six Cannabis sativa chemotypes, including 14 plants of distinct genotypes, and two extracts was determined quantitatively via high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). This represents the first comprehensive quantitative screening for 1 from various C. sativa chemotypes. Compound 1 was detected in all 13 inflorescence samples originating from “(−)- trans -Δ 9 -tetrahydrocannabinol (Δ 9 -THC, 2 ) dominant” C. sativa chemotypes, but was not detected in a “cannabidiol (CBD, 3 ) dominant” chemotype. The inflorescence content of 1 ranged approximately from 0.0023% to 0.0136% (w/w). Comprehensive inflorescence sampling was performed for each specimen investigated. A trend between inflorescence cannabinoid potency and the location of which the inflorescence was sampled on the C. sativa plant was observed for the three cannabinoids tested ( 1 – 3 ). The preliminary results obtained indicate Δ 9 -THCP ( 1 ) may have a higher degree of prevalence in C. sativa inflorescence than previously estimated.