Complete Genome Sequences of Grapevine Roditis Leaf Discoloration‐Associated Virus Identified in Turkiye

Badnavirus decoloratiovitis , commonly known as Grapevine Roditis leaf discoloration‐associated virus (GRLDaV), is a member of the genus Badnavirus (family Caulimoviridae ) and a double‐stranded DNA pathogen associated with leaf discoloration symptoms in grapevine. In this study, the complete genome sequences and molecular characterization of two GRLDaV isolates, TR‐B42 and TR‐I3, obtained from grapevine varieties in Turkiye were determined. The complete genomes were amplified in nine overlapping fragments and assembled into circular double‐stranded DNA molecules of 7084 nt (TR‐B42) and 6987 nt (TR‐I3), with G + C contents of 41.2% and 41.5%, respectively. Both isolates exhibited the typical Badnavirus genome organization comprising four open reading frames (ORFs). Conserved domain analysis revealed a DUF1319 domain in ORF1 and characteristic Badnavirus replication domains, reverse transcriptase (RT), RNase H, zinc finger, and aspartic protease, within ORF3. Independent intrinsic‐disorder prediction of ORF3 across the six globally available GRLDaV isolates converged on a moderate‐to‐high disorder window localized to ORF3 amino acids ~830–890. This same interdomain window overlapped directly with the single prominent recombination hotspot detected in the TR‐B42 genome at nucleotides 3600–3800. Quantitative dN/dS analysis showed that ORF1‐3 were each under strong purifying selection, whereas ORF4 exhibited relaxed purifying selection. Phylogenetic and pairwise comparisons showed that the Turkish isolates clustered within the GRLDaV group and shared 85%–90% complete nucleotide identity with isolates from Greece, Italy, Croatia, and Argentina, confirming their classification as GRLDaV. Building on previous analysis of GRLDaV genetic diversity and population structure in Turkish vineyards using partial genome sequences, this study presents the first complete genome sequences of GRLDaV isolates from grapevines in Turkiye.