Objective: During the preparation of Zishen Yutai Pills, 5-Hydroxymethylfurfural may be used. To establish a HPLC method for determination of 5-HMF in Zishen Yutai pill and to provide the potential quality control standard of it. Methods: RP-HPLC method was applied with the chromatographic condition as follows: the chromatographic column was Phenomenex Gemini C18 column (250×4.6mm, 5μm), methanol-water (10:90) as the mobile phrase, the flow rate was 0.8mL/min, the detection wavelength was 284nm, the column temperature was 30°C, and the injection volume was 10μL. Results: The calibration curve was linear in the range of 2.16~43.27μg. mL-1 for 5-HMF (r2=0.9993). The RSD of the method repeatability was 0.97% (n=6) and the average recovery was 99.4% (RSD=0.58%). What’s more, the specificity, precision and stability results were also good. Conclusion: The method for HPLC is not only sensitive and accurate. It is also easy to operate which can be used as the determination method for 5-HMF and has a guiding role for the quality control of the Zishen Yutai pill.
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Shaozhu et al. (2021) studied this question.
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