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A reversed-phase C30 high-performance liquid chromatography (HPLC) method was used to separate the lutein isomers of marigold flowers. The pigments were extracted from marigold meal by an Association of Official Analytical Chemists (AOAC) modified method. The unsaponified extract showed the presence of several lutein ester isomers and after saponification four major lutein isomers were detected. The relationship between all-trans-lutein, assessed by HPLC, and total xanthophylls, assessed by AOAC, was determined. A regression equation, with a high correlation coefficient (r2=0·972), which allows for the calculation of all-trans-lutein from total xanthophylls content is proposed.
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Delgado‐Vargas et al. (1996) studied this question.
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