Key result
Retrograde Langendorff perfusion of 10-day-old mouse hearts via a 26-gauge cannula achieved a mean aortic perfusion pressure of 47.9 mmHg, heart rate of 226 bpm, and contractile force of 7.2 g.
Why the study?
The small size and fragility of the mouse heart had limited the Langendorff-perfused model to adult mice, leaving an isolated-perfused newborn murine heart model unestablished to study cardiac development.
Answers are grounded in this paper and cite their sources.
The Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of the mouse heart pose significant challenges, limiting application of the Langendorff model to the study of adult mice. Cardiac development is a complex and dynamic process that is incompletely understood. Thus, establishing an isolated-perfused heart model in the newborn mouse would be an important and necessary advance. Here we present a method to successfully cannulate and perfuse the isolated newborn murine heart. We describe the basic and fundamental physiological characteristics of the ex-vivo retrograde-perfused beating neonatal heart in wild-type C57Bl/6 male mice. Our approach will enable future study of the physiological and pharmacological responses of the isolated immature murine heart to enhance knowledge of how developmental cardiac biology impacts health and disease. •The Langendorff model is a powerful tool to study the heart without confounders. •An isolated-perfused newborn murine heart model has yet to be established. •We demonstrate the first successful isolated neonatal murine heart preparation.
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Barajas et al. (2020) studied Developmental cardiac biology in isolated newborn murine heart (n=15). Isolated retrograde-perfused newborn mouse heart preparation (Langendorff model using a 26-gauge blunt aortic cannula) was evaluated on Aortic perfusion pressure (mmHg). Retrograde Langendorff perfusion of 10-day-old mouse hearts via a 26-gauge cannula achieved a mean aortic perfusion pressure of 47.9 mmHg, heart rate of 226 bpm, and contractile force of 7.2 g.
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