Time-series snRNA-seq of Brassica ovules reveals mechanism of embryo abortion in interspecific hybrids.

We have employed a single cell sequencing approach using 10x Genomics snRNAseq to comprehensively investigate the potential molecular mechanisms underlying hybrid barriers. CKs are considered to normal ovules from the intraspecies cross between B. campestris and B. campestris, while treatment group are performed on crossing ovules between B. campestris and B. oleracea over a series of time points, This study provides new insights into Brassica interspecific crossing and will facilitate the advancement of distant hybridization breeding.