This protocol describes the physicochemical characterization of amine-modified (positively charged) and carboxylate-modified (negatively charged) fluorescent polystyrene nanoparticles (PS-NPs). The purpose is to determine particle size distribution, surface charge (z-potential), morphology, and fluorescence properties prior to biological experiments. These parameters are critical for interpreting uptake behavior and toxicity.
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Luisa Diomede (2026) studied this question.
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