Cytotoxicity and biodistribution of nanoparticles in C. elegans (compiled protocols for polystyrene nanoparticles) v1

Compiled document that groups four protocols on polystyrene nanoparticles (PS-NPs): (1) physicochemical characterization of amine- and carboxylate-modified fluorescent PS-NPs; (2) cell growth and cytotoxicity in HEK 293 cells; (3) imaging of NP uptake and the effect of the protein corona; and (4) a C. elegans study assessing acute toxicity, biodistribution, reproduction, development, pharyngeal activity and locomotion of PS-NPs in Caenorhabditis elegans.